Aflatoxin and ochratoxin A were produced from Aspergillus parasiticus and Aspergillus ochraceus respectively and\r\nquantified using TLC. The toxins were mixed with broiler feed to attain required concentration. Four diets for broilers were\r\nprepared, diet 1-basal diet (control), diet 2-basal diet + aflatoxin (1 ppm) + ochratoxin (2 ppm), diet 3-basal diet+ aflatoxin (1\r\nppm) + ochratoxin (2 ppm) + activated charcoal (0.4%), diet 4- basal diet + aflatoxin (1 ppm) + ochratoxin (2 ppm) + activated\r\ncharcoal (0.4%) + yeast culture (0.2%). These four diets were fed to 4 groups of day old broiler chicks with four replicates of\r\neight birds in each replicate, in a completely randomized design for six weeks. Degenerative changes in Liver, kidney and\r\ngerminal centre depletion in spleen, cystic space in bursa of fabricius and disruption of cardiac muscle fibres were observed on\r\nhistopathology in birds fed with aflatoxin and ochratoxin supporting the biochemical study. Activated charcoal (0.4%) showed\r\nonly partial protective effect on combined toxicity in broilers. These results indicate that aflatoxin and ochratoxin in the diet\r\ncaused damage to vital organs. Though the combination of activated charcoal and yeast culture was more effective in\r\ncounteracting the combined toxicity of aflatoxin and ochratoxin compared to the activated charcoal alone, they also could not\r\ncompletely ameliorate the combined toxicity in broilers.
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